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acumen explorer ex3 laser scanning microplate cytometer  (SPT Labtech)


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    SPT Labtech acumen explorer ex3 laser scanning microplate cytometer
    Acumen Explorer Ex3 Laser Scanning Microplate Cytometer, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acumen explorer ex3 laser scanning microplate cytometer/product/SPT Labtech
    Average 86 stars, based on 1 article reviews
    acumen explorer ex3 laser scanning microplate cytometer - by Bioz Stars, 2026-05
    86/100 stars

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    acumen explorer ex3 laser scanning microplate cytometer  (SPT Labtech)
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    Acumen Explorer Ex3 Laser Scanning Microplate Cytometer, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acumen explorer ex3 laser scanning microplate cytometer/product/SPT Labtech
    Average 86 stars, based on 1 article reviews
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    acumen microplate cytometer  (SPT Labtech)
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    Three treatments are compared to assess changes in survival of dissociated hESCs: 0.1% DMSO (negative control; A,D ), 10 µM HA-1077 (positive control; B,E ), and 10 µM AST 5588603 (representative of small molecules identified in OCT4-GFP screening assay; C,F ). A-C: Readout from the Acumen <t>microplate</t> cytometer. The wells treated with HA-1077 and AST 5588603 had similar numbers of GFP positive objects (quantified in G ). D-F: Alkaline phosphatase staining of cells treated as described. Pluripotent cells are stained with a red color. Wells treated with HA-1077 ( E ) contain significantly more pluripotent cells than cells treated with DMSO or any other candidate compounds. G: Quantification of GFP signal detected in ( A- C). H: Quantification of alkaline phosphatase staining in ( D-F ) by calculating what percent of the well area stained positive for alkaline phosphatase. Data shown as mean ±SD, ***: p<0.001.
    Acumen Microplate Cytometer, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acumen microplate cytometer/product/SPT Labtech
    Average 86 stars, based on 1 article reviews
    acumen microplate cytometer - by Bioz Stars, 2026-05
    86/100 stars
    		  Buy from Supplier
    acumen ex3 microplate cytometer  (SPT Labtech)
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    SPT Labtech acumen ex3 microplate cytometer
    Three treatments are compared to assess changes in survival of dissociated hESCs: 0.1% DMSO (negative control; A,D ), 10 µM HA-1077 (positive control; B,E ), and 10 µM AST 5588603 (representative of small molecules identified in OCT4-GFP screening assay; C,F ). A-C: Readout from the Acumen <t>microplate</t> cytometer. The wells treated with HA-1077 and AST 5588603 had similar numbers of GFP positive objects (quantified in G ). D-F: Alkaline phosphatase staining of cells treated as described. Pluripotent cells are stained with a red color. Wells treated with HA-1077 ( E ) contain significantly more pluripotent cells than cells treated with DMSO or any other candidate compounds. G: Quantification of GFP signal detected in ( A- C). H: Quantification of alkaline phosphatase staining in ( D-F ) by calculating what percent of the well area stained positive for alkaline phosphatase. Data shown as mean ±SD, ***: p<0.001.
    Acumen Ex3 Microplate Cytometer, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acumen ex3 microplate cytometer/product/SPT Labtech
    Average 86 stars, based on 1 article reviews
    acumen ex3 microplate cytometer - by Bioz Stars, 2026-05
    86/100 stars
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    acumen explorer microplate cytometer  (SPT Labtech)
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    Three treatments are compared to assess changes in survival of dissociated hESCs: 0.1% DMSO (negative control; A,D ), 10 µM HA-1077 (positive control; B,E ), and 10 µM AST 5588603 (representative of small molecules identified in OCT4-GFP screening assay; C,F ). A-C: Readout from the Acumen <t>microplate</t> cytometer. The wells treated with HA-1077 and AST 5588603 had similar numbers of GFP positive objects (quantified in G ). D-F: Alkaline phosphatase staining of cells treated as described. Pluripotent cells are stained with a red color. Wells treated with HA-1077 ( E ) contain significantly more pluripotent cells than cells treated with DMSO or any other candidate compounds. G: Quantification of GFP signal detected in ( A- C). H: Quantification of alkaline phosphatase staining in ( D-F ) by calculating what percent of the well area stained positive for alkaline phosphatase. Data shown as mean ±SD, ***: p<0.001.
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    https://www.bioz.com/result/acumen explorer microplate cytometer/product/SPT Labtech
    Average 86 stars, based on 1 article reviews
    acumen explorer microplate cytometer - by Bioz Stars, 2026-05
    86/100 stars
    		  Buy from Supplier
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    acumen explorer laser scanning fluorescence microplate cytometer  (SPT Labtech)
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    Three treatments are compared to assess changes in survival of dissociated hESCs: 0.1% DMSO (negative control; A,D ), 10 µM HA-1077 (positive control; B,E ), and 10 µM AST 5588603 (representative of small molecules identified in OCT4-GFP screening assay; C,F ). A-C: Readout from the Acumen <t>microplate</t> cytometer. The wells treated with HA-1077 and AST 5588603 had similar numbers of GFP positive objects (quantified in G ). D-F: Alkaline phosphatase staining of cells treated as described. Pluripotent cells are stained with a red color. Wells treated with HA-1077 ( E ) contain significantly more pluripotent cells than cells treated with DMSO or any other candidate compounds. G: Quantification of GFP signal detected in ( A- C). H: Quantification of alkaline phosphatase staining in ( D-F ) by calculating what percent of the well area stained positive for alkaline phosphatase. Data shown as mean ±SD, ***: p<0.001.
    Acumen Explorer Laser Scanning Fluorescence Microplate Cytometer, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    Three treatments are compared to assess changes in survival of dissociated hESCs: 0.1% DMSO (negative control; A,D ), 10 µM HA-1077 (positive control; B,E ), and 10 µM AST 5588603 (representative of small molecules identified in OCT4-GFP screening assay; C,F ). A-C: Readout from the Acumen microplate cytometer. The wells treated with HA-1077 and AST 5588603 had similar numbers of GFP positive objects (quantified in G ). D-F: Alkaline phosphatase staining of cells treated as described. Pluripotent cells are stained with a red color. Wells treated with HA-1077 ( E ) contain significantly more pluripotent cells than cells treated with DMSO or any other candidate compounds. G: Quantification of GFP signal detected in ( A- C). H: Quantification of alkaline phosphatase staining in ( D-F ) by calculating what percent of the well area stained positive for alkaline phosphatase. Data shown as mean ±SD, ***: p<0.001.

    Journal: PLoS ONE

    Article Title: Small Molecule Screening with Laser Cytometry Can Be Used to Identify Pro-Survival Molecules in Human Embryonic Stem Cells

    doi: 10.1371/journal.pone.0054948

    Figure Lengend Snippet: Three treatments are compared to assess changes in survival of dissociated hESCs: 0.1% DMSO (negative control; A,D ), 10 µM HA-1077 (positive control; B,E ), and 10 µM AST 5588603 (representative of small molecules identified in OCT4-GFP screening assay; C,F ). A-C: Readout from the Acumen microplate cytometer. The wells treated with HA-1077 and AST 5588603 had similar numbers of GFP positive objects (quantified in G ). D-F: Alkaline phosphatase staining of cells treated as described. Pluripotent cells are stained with a red color. Wells treated with HA-1077 ( E ) contain significantly more pluripotent cells than cells treated with DMSO or any other candidate compounds. G: Quantification of GFP signal detected in ( A- C). H: Quantification of alkaline phosphatase staining in ( D-F ) by calculating what percent of the well area stained positive for alkaline phosphatase. Data shown as mean ±SD, ***: p<0.001.

    Article Snippet: In all cases, data were acquired using an Acumen microplate cytometer (TTP Labtech).

    Techniques: Negative Control, Positive Control, Screening Assay, Cytometry, Staining